Kinetic model of enzymatic synthesis of γ-D-glutamyl-L-tryptophan

A new model of enzymatic synthesis of γ-D-Glu-L-Trp (SCV-07) from D-glutamine and L-tryptophan was developed. The K_m and K_cat of transpeptidation and enzymatic hydrolysis of product (presented as K_m′ and K_cat′) were determined as K_m = 5.11 mmol·L^-1, K_cat = 3.92 mmol·min^-1, K_m′ = 2.31 mmol·L^-1 and K_cat′ = 1.46 mmol·min^-1. The sequential mechanism was used to describe the rate of transpeptidation, and the model parameters were optimized. The enzymatic synthesis of γ-D-glutamyl-L-tryptophan was investigated to verify the model. Good agreement between experimental data and calculations was obtained. The total relative errors of experimental data and calculations were less than 5%.