Abstract
The ring-opening polymerization of ε-caprolactone catalyzed by a novel lipase Candida sp. 99-125 was achieved in organic solvents. ε-Caprolactone was found to be polymerized in quantitative yield in toluene at 80 °C for 5 days. The poly (ε-caprolactone) obtained had a number-average molecular weight of 14,120 (polydispersity index = 1.161). The effect of enzyme concentration, temperature, reaction time, reaction medium, and water content on monomer conversion and product molecular weight were investigated. Kinetic analysis showed that the enzyme displayed Michaelis-Menten kinetics and showed stronger affinity for ε-caprolactone (with a Km value of 6.1 mmol/L) than other reported lipases.
| Original language | English |
|---|---|
| Pages (from-to) | 150-155 |
| Number of pages | 6 |
| Journal | Biocatalysis and Biotransformation |
| Volume | 33 |
| Issue number | 3 |
| DOIs | |
| State | Published - 4 May 2015 |
Keywords
- Candida sp. 99-125
- enzymatic catalysis
- ring-opening polymerization
- ε-caprolactone