摘要
The ring-opening polymerization of ε-caprolactone catalyzed by a novel lipase Candida sp. 99-125 was achieved in organic solvents. ε-Caprolactone was found to be polymerized in quantitative yield in toluene at 80 °C for 5 days. The poly (ε-caprolactone) obtained had a number-average molecular weight of 14,120 (polydispersity index = 1.161). The effect of enzyme concentration, temperature, reaction time, reaction medium, and water content on monomer conversion and product molecular weight were investigated. Kinetic analysis showed that the enzyme displayed Michaelis-Menten kinetics and showed stronger affinity for ε-caprolactone (with a Km value of 6.1 mmol/L) than other reported lipases.
| 源语言 | 英语 |
|---|---|
| 页（从-至） | 150-155 |
| 页数 | 6 |
| 期刊 | Biocatalysis and Biotransformation |
| 卷 | 33 |
| 期 | 3 |
| DOI | |
| 出版状态 | 已出版 - 4 5月 2015 |