Strain improvement of Rhizopus oryzae for over-production of fumaric acid by reducing ethanol synthesis pathway

Yong Qian Fu; Qing Xu; Shuang Li; Yao Chen; He Huang

doi:10.1007/s11814-009-0323-3

Strain improvement of Rhizopus oryzae for over-production of fumaric acid by reducing ethanol synthesis pathway

Yong Qian Fu, Qing Xu, Shuang Li, Yao Chen, He Huang

COLLEGE OF BIOTECHNOLOGY AND PHARMACEUTICAL ENGINEERING

Nanjing Tech University

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Rhizopus oryzae mutants were isolated from the wild type strain ME-F01 after mutagenesis with UV coupled with nitrosoguanidine (NTG) and the following allyl alcohol resistance selection method. By analyzing the activities of alcohol dehydrogenase (ADH) involved in ethanol synthesis pathway and batch fermentation, one mutant, ME-UN-8, was isolated that produced 21. 1% more fumaric acid than ME-F01 with the corresponding byproduct of ethanol decreased by 83. 7%.

Original language	English
Pages (from-to)	183-186
Number of pages	4
Journal	Korean Journal of Chemical Engineering
Volume	27
Issue number	1
DOIs	https://doi.org/10.1007/s11814-009-0323-3
State	Published - Jan 2010

Keywords

Alcohol Dehydrogenase
Allyl Alcohol
Fumaric Acid
Mutation
Rhizopus oryzae

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10.1007/s11814-009-0323-3

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title = "Strain improvement of Rhizopus oryzae for over-production of fumaric acid by reducing ethanol synthesis pathway",

abstract = "Rhizopus oryzae mutants were isolated from the wild type strain ME-F01 after mutagenesis with UV coupled with nitrosoguanidine (NTG) and the following allyl alcohol resistance selection method. By analyzing the activities of alcohol dehydrogenase (ADH) involved in ethanol synthesis pathway and batch fermentation, one mutant, ME-UN-8, was isolated that produced 21. 1% more fumaric acid than ME-F01 with the corresponding byproduct of ethanol decreased by 83. 7%.",

keywords = "Alcohol Dehydrogenase, Allyl Alcohol, Fumaric Acid, Mutation, Rhizopus oryzae",

author = "Fu, {Yong Qian} and Qing Xu and Shuang Li and Yao Chen and He Huang",

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AU - Fu, Yong Qian

AU - Xu, Qing

AU - Li, Shuang

AU - Chen, Yao

AU - Huang, He

PY - 2010/1

Y1 - 2010/1

N2 - Rhizopus oryzae mutants were isolated from the wild type strain ME-F01 after mutagenesis with UV coupled with nitrosoguanidine (NTG) and the following allyl alcohol resistance selection method. By analyzing the activities of alcohol dehydrogenase (ADH) involved in ethanol synthesis pathway and batch fermentation, one mutant, ME-UN-8, was isolated that produced 21. 1% more fumaric acid than ME-F01 with the corresponding byproduct of ethanol decreased by 83. 7%.

AB - Rhizopus oryzae mutants were isolated from the wild type strain ME-F01 after mutagenesis with UV coupled with nitrosoguanidine (NTG) and the following allyl alcohol resistance selection method. By analyzing the activities of alcohol dehydrogenase (ADH) involved in ethanol synthesis pathway and batch fermentation, one mutant, ME-UN-8, was isolated that produced 21. 1% more fumaric acid than ME-F01 with the corresponding byproduct of ethanol decreased by 83. 7%.

KW - Alcohol Dehydrogenase

KW - Allyl Alcohol

KW - Fumaric Acid

KW - Mutation

KW - Rhizopus oryzae

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SN - 0256-1115

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JO - Korean Journal of Chemical Engineering

JF - Korean Journal of Chemical Engineering

IS - 1

ER -

Strain improvement of Rhizopus oryzae for over-production of fumaric acid by reducing ethanol synthesis pathway

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Keywords

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