Strain improvement of Rhizopus oryzae for over-production of fumaric acid by reducing ethanol synthesis pathway

Yong Qian Fu; Qing Xu; Shuang Li; Yao Chen; He Huang

doi:10.1007/s11814-009-0323-3

Strain improvement of Rhizopus oryzae for over-production of fumaric acid by reducing ethanol synthesis pathway

Yong Qian Fu, Qing Xu, Shuang Li, Yao Chen, He Huang

生物与制药工程学院

Nanjing Tech University

科研成果: 期刊稿件 › 文章 › 同行评审

30 引用（Scopus）

摘要

Rhizopus oryzae mutants were isolated from the wild type strain ME-F01 after mutagenesis with UV coupled with nitrosoguanidine (NTG) and the following allyl alcohol resistance selection method. By analyzing the activities of alcohol dehydrogenase (ADH) involved in ethanol synthesis pathway and batch fermentation, one mutant, ME-UN-8, was isolated that produced 21. 1% more fumaric acid than ME-F01 with the corresponding byproduct of ethanol decreased by 83. 7%.

源语言	英语
页（从-至）	183-186
页数	4
期刊	Korean Journal of Chemical Engineering
卷	27
期	1
DOI	https://doi.org/10.1007/s11814-009-0323-3
出版状态	已出版 - 1月 2010

访问文件

10.1007/s11814-009-0323-3

其它文件与链接

链接到 Scopus 的出版物

引用此

@article{2364c9ee1dde4518a1e26a9ee333990b,

title = "Strain improvement of Rhizopus oryzae for over-production of fumaric acid by reducing ethanol synthesis pathway",

abstract = "Rhizopus oryzae mutants were isolated from the wild type strain ME-F01 after mutagenesis with UV coupled with nitrosoguanidine (NTG) and the following allyl alcohol resistance selection method. By analyzing the activities of alcohol dehydrogenase (ADH) involved in ethanol synthesis pathway and batch fermentation, one mutant, ME-UN-8, was isolated that produced 21. 1% more fumaric acid than ME-F01 with the corresponding byproduct of ethanol decreased by 83. 7%.",

keywords = "Alcohol Dehydrogenase, Allyl Alcohol, Fumaric Acid, Mutation, Rhizopus oryzae",

author = "Fu, {Yong Qian} and Qing Xu and Shuang Li and Yao Chen and He Huang",

year = "2010",

month = jan,

doi = "10.1007/s11814-009-0323-3",

language = "英语",

volume = "27",

pages = "183--186",

journal = "Korean Journal of Chemical Engineering",

issn = "0256-1115",

publisher = "Springer",

number = "1",

}

TY - JOUR

T1 - Strain improvement of Rhizopus oryzae for over-production of fumaric acid by reducing ethanol synthesis pathway

AU - Fu, Yong Qian

AU - Xu, Qing

AU - Li, Shuang

AU - Chen, Yao

AU - Huang, He

PY - 2010/1

Y1 - 2010/1

N2 - Rhizopus oryzae mutants were isolated from the wild type strain ME-F01 after mutagenesis with UV coupled with nitrosoguanidine (NTG) and the following allyl alcohol resistance selection method. By analyzing the activities of alcohol dehydrogenase (ADH) involved in ethanol synthesis pathway and batch fermentation, one mutant, ME-UN-8, was isolated that produced 21. 1% more fumaric acid than ME-F01 with the corresponding byproduct of ethanol decreased by 83. 7%.

AB - Rhizopus oryzae mutants were isolated from the wild type strain ME-F01 after mutagenesis with UV coupled with nitrosoguanidine (NTG) and the following allyl alcohol resistance selection method. By analyzing the activities of alcohol dehydrogenase (ADH) involved in ethanol synthesis pathway and batch fermentation, one mutant, ME-UN-8, was isolated that produced 21. 1% more fumaric acid than ME-F01 with the corresponding byproduct of ethanol decreased by 83. 7%.

KW - Alcohol Dehydrogenase

KW - Allyl Alcohol

KW - Fumaric Acid

KW - Mutation

KW - Rhizopus oryzae

UR - http://www.scopus.com/inward/record.url?scp=74249124114&partnerID=8YFLogxK

U2 - 10.1007/s11814-009-0323-3

DO - 10.1007/s11814-009-0323-3

M3 - 文章

AN - SCOPUS:74249124114

SN - 0256-1115

VL - 27

SP - 183

EP - 186

JO - Korean Journal of Chemical Engineering

JF - Korean Journal of Chemical Engineering

IS - 1

ER -

Strain improvement of Rhizopus oryzae for over-production of fumaric acid by reducing ethanol synthesis pathway

摘要

访问文件

其它文件与链接

指纹

引用此