TY - JOUR
T1 - A Nanozymatic-Mediated Smartphone Colorimetric Sensing Platform for the Detection of Dimethyl Phthalate (DMP) and Dibutyl Phthalate (DBP)
AU - Li, Wenhui
AU - Zhang, Xuecheng
AU - Zhang, Haojie
AU - Zhang, Cheng
AU - Chen, Yingjie
AU - Li, Cong
AU - Hu, Yonghong
AU - Yu, Xiaoping
AU - Zhang, Biao
AU - Lin, Xiaodong
N1 - Publisher Copyright:
© 2023 by the authors.
PY - 2023/10
Y1 - 2023/10
N2 - Plasticizers are a type of toxic substance that may remain in food, posing significant health risks including carcinogenic, teratogenic, mutagenic, and other adverse effects. In this study, a novel strategy was employed by combining Pt@Au nanozymes with high catalytic properties to created two catalytic signal probes, designated as Pt@Au@Ab1 and Pt@Au@Ab2, specifically designed for the detection of dimethyl phthalate (DMP) and dibutyl phthalate (DBP). These catalytic signal probes served as the foundation for the development of a colorimetric immunoassay, enabling the simultaneous detection of both DMP and DBP. The colorimetric immunoassay is capable of detecting DMP in the range of 0.5–100 μg/L with a limit of detection as low as 0.1 μg/L and DBP in the range of 1–32 μg/L with a low limit of detection of 0.5 μg/L. The developed immunoassay can be used for the determination of the DMP and DBP in baijiu and plastic bottled drinks. The recovery rate is in the range of 96.4% and 100.5% and the coefficient of variation is between 1.0% and 7.2%. This innovative colorimetric immunoassay offers a robust tool for the simultaneous quantification of DMP and DBP in real samples.
AB - Plasticizers are a type of toxic substance that may remain in food, posing significant health risks including carcinogenic, teratogenic, mutagenic, and other adverse effects. In this study, a novel strategy was employed by combining Pt@Au nanozymes with high catalytic properties to created two catalytic signal probes, designated as Pt@Au@Ab1 and Pt@Au@Ab2, specifically designed for the detection of dimethyl phthalate (DMP) and dibutyl phthalate (DBP). These catalytic signal probes served as the foundation for the development of a colorimetric immunoassay, enabling the simultaneous detection of both DMP and DBP. The colorimetric immunoassay is capable of detecting DMP in the range of 0.5–100 μg/L with a limit of detection as low as 0.1 μg/L and DBP in the range of 1–32 μg/L with a low limit of detection of 0.5 μg/L. The developed immunoassay can be used for the determination of the DMP and DBP in baijiu and plastic bottled drinks. The recovery rate is in the range of 96.4% and 100.5% and the coefficient of variation is between 1.0% and 7.2%. This innovative colorimetric immunoassay offers a robust tool for the simultaneous quantification of DMP and DBP in real samples.
KW - Pt@Au nanozyme
KW - colorimetric immunoassay
KW - dibutyl phthalate (DBP)
KW - dimethyl phthalate (DMP)
KW - two-component simultaneous detection
UR - http://www.scopus.com/inward/record.url?scp=85175095026&partnerID=8YFLogxK
U2 - 10.3390/bios13100919
DO - 10.3390/bios13100919
M3 - 文章
C2 - 37887112
AN - SCOPUS:85175095026
SN - 2079-6374
VL - 13
JO - Biosensors
JF - Biosensors
IS - 10
M1 - 919
ER -
