摘要
Kinetic model of conversion process for the production of D-N-carbamoyl phenylalanine from benzylhydantoin by hydantoinase purified from Burkholderia cepecia njutl was developed. The study indicated important impact of substrate racemization on enzymatic conversion. The influence of temperature on bioconversion was discussed. The values of solubility and solution constant, racemization constant and Michaelis constant were determined at different temperatures. Temperature was important in conversion of benzylhydantoin and increasing thermostability of hydantoinase was significant for production of D-amino acids.
| 源语言 | 英语 |
|---|---|
| 页（从-至） | 629-634 |
| 页数 | 6 |
| 期刊 | Huagong Xuebao/CIESC Journal |
| 卷 | 55 |
| 期 | 4 |
| 出版状态 | 已出版 - 4月 2004 |