Separation of S-benzyl-γ-L-glutamyl-L-cysteine using ion-exchange method

The synthesized S-benzyl-γ-L-glutarhyl-L-cysteine (S-Bzl-GGC) with enzymatic methods was purified with ion-exchange method by using Q sepharose FF as adsorbent. The adsorption isotherm was investigated, and Sips equation provided the best fitting of experimental data. The influences of pH value, elution gradient, flow rate and sample quantity on the separation process were also discussed. The results showed that S-Bzl-GGC could be well separated at the conditions of pH 8.2, salt elution gradient of 0-30% B, 12 mL, flow rate of 2 mL/min and sample size of 500 μL. Under the optimal conditions, the purity of product was 98.5% after one step purification and the structure of product was further identified by ¹H-NMR.