Fabrication of high-density protein microarray on an EDTA-PDMS substrate by microcontact printing

Haijun Yang; Hai Li; Peng Wang; Bin Li

Fabrication of high-density protein microarray on an EDTA-PDMS substrate by microcontact printing

Haijun Yang, Hai Li, Peng Wang, Bin Li

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

In this paper, a new and facile route was developed to efficiently print protein microarrays onto hydrophobic polydimethylsiloxane (PDMS) substrate. The unique feature of this method was that protein could be easily transferred onto PDMS substrate by first physically adsorbing EDTA molecules on the PDMS surface. AFM results showed that the transferred patterns are of high fidelity and density with average width of about 500 nm and the interval of 1 μm.

Original language	English
Pages (from-to)	946-950
Number of pages	5
Journal	He Jishu/Nuclear Techniques
Volume	31
Issue number	12
State	Published - Dec 2008
Externally published	Yes

Keywords

AFM
Microcontact printing
Physical adsorption
Polydimethylsiloxane
Protein microarray

Cite this

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title = "Fabrication of high-density protein microarray on an EDTA-PDMS substrate by microcontact printing",

abstract = "In this paper, a new and facile route was developed to efficiently print protein microarrays onto hydrophobic polydimethylsiloxane (PDMS) substrate. The unique feature of this method was that protein could be easily transferred onto PDMS substrate by first physically adsorbing EDTA molecules on the PDMS surface. AFM results showed that the transferred patterns are of high fidelity and density with average width of about 500 nm and the interval of 1 μm.",

keywords = "AFM, Microcontact printing, Physical adsorption, Polydimethylsiloxane, Protein microarray",

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AU - Li, Hai

AU - Wang, Peng

AU - Li, Bin

PY - 2008/12

Y1 - 2008/12

N2 - In this paper, a new and facile route was developed to efficiently print protein microarrays onto hydrophobic polydimethylsiloxane (PDMS) substrate. The unique feature of this method was that protein could be easily transferred onto PDMS substrate by first physically adsorbing EDTA molecules on the PDMS surface. AFM results showed that the transferred patterns are of high fidelity and density with average width of about 500 nm and the interval of 1 μm.

AB - In this paper, a new and facile route was developed to efficiently print protein microarrays onto hydrophobic polydimethylsiloxane (PDMS) substrate. The unique feature of this method was that protein could be easily transferred onto PDMS substrate by first physically adsorbing EDTA molecules on the PDMS surface. AFM results showed that the transferred patterns are of high fidelity and density with average width of about 500 nm and the interval of 1 μm.

KW - AFM

KW - Microcontact printing

KW - Physical adsorption

KW - Polydimethylsiloxane

KW - Protein microarray

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M3 - 文章

AN - SCOPUS:58249088824

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JO - He Jishu/Nuclear Techniques

JF - He Jishu/Nuclear Techniques

IS - 12

ER -

Fabrication of high-density protein microarray on an EDTA-PDMS substrate by microcontact printing

Abstract

Keywords

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