TY - JOUR
T1 - Phosphoenolpyruvate-dependent phosphorylation of sucrose by Clostridium tyrobutyricum ZJU 8235
T2 - Evidence for the phosphotransferase transport system
AU - Jiang, Ling
AU - Cai, Jin
AU - Wang, Jufang
AU - Liang, Shizhong
AU - Xu, Zhinan
AU - Yang, Shang Tian
PY - 2010/1
Y1 - 2010/1
N2 - The uptake and metabolism of sucrose, the major sugar in industrial cane molasses, by Clostridium tyrobutyricum ZJU 8235 was investigated and this study provided the first definitive evidence for phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) activity in butyric acid-producing bacteria. Glucose was utilized preferentially to sucrose when both substrates were present in the medium. The PEP-dependent sucrose: PTS was induced by growing C. tyrobutyricum on sucrose (but not glucose) as the sole carbon source. Extract fractionation and PTS reconstitution experiments revealed that both soluble and membrane components were required for bioactivity. Sucrose-6-phosphate hydrolase and fructokinase activities were also detected in sucrose-grown cultures. Based on these findings, a pathway of sucrose metabolism in this organism was proposed that includes the forming of sucrose-6-phosphate via the PTS and its further degradation into glucose-6-phosphate and fructose-6-phosphate.
AB - The uptake and metabolism of sucrose, the major sugar in industrial cane molasses, by Clostridium tyrobutyricum ZJU 8235 was investigated and this study provided the first definitive evidence for phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) activity in butyric acid-producing bacteria. Glucose was utilized preferentially to sucrose when both substrates were present in the medium. The PEP-dependent sucrose: PTS was induced by growing C. tyrobutyricum on sucrose (but not glucose) as the sole carbon source. Extract fractionation and PTS reconstitution experiments revealed that both soluble and membrane components were required for bioactivity. Sucrose-6-phosphate hydrolase and fructokinase activities were also detected in sucrose-grown cultures. Based on these findings, a pathway of sucrose metabolism in this organism was proposed that includes the forming of sucrose-6-phosphate via the PTS and its further degradation into glucose-6-phosphate and fructose-6-phosphate.
KW - Clostridium tyrobutyricum
KW - Phosphotransferase system
KW - Sucrose metabolism
UR - http://www.scopus.com/inward/record.url?scp=70249148517&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2009.08.024
DO - 10.1016/j.biortech.2009.08.024
M3 - 文章
C2 - 19726178
AN - SCOPUS:70249148517
SN - 0960-8524
VL - 101
SP - 304
EP - 309
JO - Bioresource Technology
JF - Bioresource Technology
IS - 1
ER -
