Phosphoenolpyruvate-dependent phosphorylation of sucrose by Clostridium tyrobutyricum ZJU 8235: Evidence for the phosphotransferase transport system

Ling Jiang, Jin Cai, Jufang Wang, Shizhong Liang, Zhinan Xu, Shang Tian Yang

科研成果: 期刊稿件文章同行评审

26 引用 (Scopus)

摘要

The uptake and metabolism of sucrose, the major sugar in industrial cane molasses, by Clostridium tyrobutyricum ZJU 8235 was investigated and this study provided the first definitive evidence for phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) activity in butyric acid-producing bacteria. Glucose was utilized preferentially to sucrose when both substrates were present in the medium. The PEP-dependent sucrose: PTS was induced by growing C. tyrobutyricum on sucrose (but not glucose) as the sole carbon source. Extract fractionation and PTS reconstitution experiments revealed that both soluble and membrane components were required for bioactivity. Sucrose-6-phosphate hydrolase and fructokinase activities were also detected in sucrose-grown cultures. Based on these findings, a pathway of sucrose metabolism in this organism was proposed that includes the forming of sucrose-6-phosphate via the PTS and its further degradation into glucose-6-phosphate and fructose-6-phosphate.

源语言英语
页(从-至)304-309
页数6
期刊Bioresource Technology
101
1
DOI
出版状态已出版 - 1月 2010
已对外发布

指纹

探究 'Phosphoenolpyruvate-dependent phosphorylation of sucrose by Clostridium tyrobutyricum ZJU 8235: Evidence for the phosphotransferase transport system' 的科研主题。它们共同构成独一无二的指纹。

引用此