纤维二糖差向异构酶的研究与应用概述

Cellobiose 2-epimerase has attracted growing attentions as an industrial enzyme. It was applied to lactulose bio-production, as well as D-mannose preparation. Lactulose was used for treating constipation and hepatic encephalopathy as a common over-the-counter drug, and showing a high efficiency. The annual global demand for lactulose was nearly 100 000 tons, showing great commercial value. In this review, recent advances in new enzyme characterization, protein engineering and enzyme immobilization for cellobiose 2-epimerase are summarized, which would shed light on researches in this field.