荧光内滤效应技术在生物检测和疾病标志上的应用

As an important non-irradiation energy conversion model, the mechanism of inner filter effect(IFE) is that the absorption spectrum of the absorbers overlaps with the excitation spectrum or emission spectrum or both spectra of the fluorophor, resulting in the fluorescence quenching of the excitation peak/emission peak of the fluorophor. The suitable donor-acceptor pair is a significant factor for successfully constructing IFE sensors to detect targets. In recent years, IFE technology has been widely studied by researchers because of its simple operation, high sensitivity, no need to modify donor and link donor and receptor. Disease is a serious threat to people's health. Early diagnosis and early treatment are the most effective ways to prevent disease and protect health. This review summarizes the research results of IFE technology in recent years for detecting enzyme activities, pesticides, metabolites and small molecular chemicals on disease markers and health monitoring, and explains the unique design of IFE sensors based on "turn-off", "turn-on" and "ratiometric fluorescence assay" method. The advantages and disadvantages of each method are also discussed. Finally, the advantages and practical obstacles of IFE technology in disease labeling and health monitoring are briefly pointed out, and the prospects for the future development of IFE technology and disease surveillance methods are also prospected.