TY - JOUR
T1 - Efficient degradation of the polysaccharide extracted from Enteromorpha prolifera by using a novel polysaccharide lyase family 28 enzyme with high activity
AU - Zhu, Benwei
AU - Li, Chen
AU - Yao, Zhong
AU - Xu, Hong
AU - Ning, Limin
N1 - Publisher Copyright:
© 2024 Elsevier Ltd
PY - 2025/2/1
Y1 - 2025/2/1
N2 - The polysaccharides originated from Enteromorpha species exhibited versatile physiological activities and great potential in food and medicine industries. The oligosaccharides, which prepared from polysaccharide by enzymatic hydrolysis, retained the excellent activity as polysaccharide, and then revealed better solubility, bioavailability and effectiveness. However, there are few reports on Enteromorpha polysaccharide (EP)-degrading enzymes for efficient degradation of EP and high-valued utilization of Enteromorpha biomass. Herein, a novel EP-degrading enzyme, EPD1, was identified and heterologously expressed. It could efficiently hydrolyze the EP with high activity (985.755 U/mg) and exhibited optimal activity at 50 °C and a pH of 10.0. The Km value of EPD1 was 7.5512 mg·mL−1 and the Vmax value was 4.9109 μmol·min−1·mL−1. Furthermore, EPD1 demonstrated cold adaptation as evidenced by minimal activity loss following incubation at temperatures below 30 °C for 1 h. HPLC and ESI-MS analysis revealed that EPD1 could produce disaccharides, trisaccharides and tetrasaccharides as the final degradation products from EPs. In conclusion, a novel EP-degrading enzyme with high activity and excellent performance was identified and it can expand the database of EP-degrading enzymes and provide the possibility to make full use of EPs.
AB - The polysaccharides originated from Enteromorpha species exhibited versatile physiological activities and great potential in food and medicine industries. The oligosaccharides, which prepared from polysaccharide by enzymatic hydrolysis, retained the excellent activity as polysaccharide, and then revealed better solubility, bioavailability and effectiveness. However, there are few reports on Enteromorpha polysaccharide (EP)-degrading enzymes for efficient degradation of EP and high-valued utilization of Enteromorpha biomass. Herein, a novel EP-degrading enzyme, EPD1, was identified and heterologously expressed. It could efficiently hydrolyze the EP with high activity (985.755 U/mg) and exhibited optimal activity at 50 °C and a pH of 10.0. The Km value of EPD1 was 7.5512 mg·mL−1 and the Vmax value was 4.9109 μmol·min−1·mL−1. Furthermore, EPD1 demonstrated cold adaptation as evidenced by minimal activity loss following incubation at temperatures below 30 °C for 1 h. HPLC and ESI-MS analysis revealed that EPD1 could produce disaccharides, trisaccharides and tetrasaccharides as the final degradation products from EPs. In conclusion, a novel EP-degrading enzyme with high activity and excellent performance was identified and it can expand the database of EP-degrading enzymes and provide the possibility to make full use of EPs.
KW - Biochemical characterization
KW - Enteromorpha polysaccharide
KW - EP-degrading enzymes
UR - http://www.scopus.com/inward/record.url?scp=85206832318&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2024.141725
DO - 10.1016/j.foodchem.2024.141725
M3 - 文章
C2 - 39442213
AN - SCOPUS:85206832318
SN - 0308-8146
VL - 464
JO - Food Chemistry
JF - Food Chemistry
M1 - 141725
ER -