Dicyanisophorone-based near-infrared fluorescent probe for the detection of thiophenol and its application in living cells and actual water samples

This article reports a new type of dicyanisophorone-based near-infrared fluorescent probe for the rapid detection of mercaptophenol by introducing 2,4-dinitrobenzene sulfonate group as a specific recognition group for thiophenol. The probe has a significant large Stokes shift (185 nm). At the same time, it exhibits rapid response, high selectivity and high sensitivity to thiophene. In addition, the fluorescence of the probe at 650 nm has a good linear relationship with the concentration of thiophenol in the range of 0–100 μM, and the detection limit is as low as 65 nM. The probe has been successfully applied to the detection of thiophenol in actual water samples, and has good live cell imaging effects, and at the same time shows the superiority of its low cell toxicity.