Improvement of poly(γ-glutamic acid) biosynthesis and redistribution of metabolic flux with the presence of different additives in Bacillus subtilis CGMCC 0833

Qun Wu, Hong Xu, Ningning Shi, Jun Yao, Sha Li, Pingkai Ouyang

科研成果: 期刊稿件文章同行评审

58 引用 (Scopus)

摘要

Tween-80, dimethyl sulfoxide (DMSO), and glycerol could be used as novel materials to regulate the central carbon metabolic pathway and improve γ-PGA biosynthesis by Bacillus subtilis CGMCC 0833. With glycerol in the medium, the activity of 2-oxoglutarate dehydrogenase complex at the key node of 2-oxoglutarate was depressed, more carbon flux distribution was directed to synthesize glutamate, the substrate of γ-PGA, which led to overproducing of γ-PGA, reached 31.7 g/l, compared to the original value of 26.7 g/l. When Tween-80 or DMSO was in the medium, the activity of isocitrate dehydrogenase was stimulated, the branch flux from 2-oxoglutarate to glutamate was also enhanced due to the increasing of total flux from iso-citrate to 2-oxoglutarate, then a large amount of glutamate was produced, and formation of γ-PGA was also improved, which was a different process compared with that of glycerol. Moreover, with the addition of Tween-80 or DMSO, cell membrane permeability was increased, which facilitated the uptake of extracellular substrates and the secretion of γ-PGA by this strain; therefore, γ-PGA production was further stimulated, and 34.4 and 32.7 g/l γ-PGA were obtained, respectively. This work firstly employed additives to improve the biosynthesis of γ-PGA and would be helpful in understanding the biosynthesis mechanism of γ-PGA by Bacillus species deeply.

源语言英语
页(从-至)527-535
页数9
期刊Applied Microbiology and Biotechnology
79
4
DOI
出版状态已出版 - 6月 2008

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